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词汇 Southern blot
释义

Definition of Southern blot in English:

Southern blot

noun
Biology
  • A procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which assay by hybridization may be carried out.

    〔生〕DNA印迹

    Example sentencesExamples
    • In Southern blots, DNA is cut with restriction enzymes then separated.
    • Standard molecular biological procedures, such as Southern blots, were performed as described.
    • Columns of horizontal ovals and rectangles indicate lack of hybridization on the Southern blots.
    • Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing.
    • The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed.

Origin

1970s: named after Edwin M. Southern (born 1938), British biochemist.

Definition of Southern blot in US English:

Southern blot

noun
Biology
  • A procedure for identifying specific sequences of DNA, in which fragments separated on a gel are transferred directly to a second medium on which detection by hybridization may be carried out.

    〔生〕DNA印迹

    Example sentencesExamples
    • In Southern blots, DNA is cut with restriction enzymes then separated.
    • Columns of horizontal ovals and rectangles indicate lack of hybridization on the Southern blots.
    • The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed.
    • Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing.
    • Standard molecular biological procedures, such as Southern blots, were performed as described.

Origin

1970s: named after Edwin M. Southern (born 1938), British biochemist.

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